The molecular mechanisms of activation of G-protein gated inwardly rectifying K+ channels (360G-Wellcome-081475_Z_06_Z)

£1,039,333

The hypothesis I would like to examine is "In atrial myocytes the inhibitory G-protein subunit nucleates the formation of a signalling complex of a dimeric G-protein coupled receptor (GPCR), for example the M2 muscarinic receptor, heterotrimeric G-protein, regulators of G-protein signalling and G-protein gated K+ channel." Specifically the goals are Goal 1:- To examine for M2 muscarinic, A1 adenosine and S1P3 sphingosine-1-phosphate receptor dimerisation and to delineate the molecular determinants and consequences of oligomerisation in native atrial myocytes. Goal 2:- To determine if a complex exists of GPCR with Gi\o-protein heterotrimers and with GIRK channels prior to agonist exposure and what happens to the complex in the presence of agonist. Goal 3:- To examine the role of individual Gi\o isoforms and RGSs, in particular RGS3, RGS4 and RGS5, in signalling to GIRK channels in native atrial myocytes.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 1039333
Applicant Surname Tinker
Approval Committee Physiological Sciences Funding Committee
Award Date 2007-02-22T00:00:00+00:00
Financial Year 2006/07
Grant Programme: Title Programme Grant
Internal ID 081475/Z/06/Z
Lead Applicant Prof Andrew Tinker
Partnership Value 1039333
Planned Dates: End Date 2011-08-31T00:00:00+00:00
Planned Dates: Start Date 2007-09-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London