Molecular mechanisms of repression mediated by Groucho family proteins. (360G-Wellcome-090021_Z_09_Z)
Control of gene expression is crucial for the development and survival of all animals. Incorrect gene expression can lead to death or severe abnormalities during development, and in adult life may cause cancer. We will focus on investigating the mechanism of transcriptional repression mediated by the evolutionarily conserved Groucho family of corepressors. Although it is well established that Groucho family proteins interact with many transcription factors to mediate repression, the molecular me chanisms through which they inhibit transcription are not well understood. We propose molecular and genetic approaches to dissect Groucho family corepressor function using Drosophila as a model system. To study the role of oligomerization of Groucho proteins in vivo, we will generate mutations that disrupt oligomerization or replace the native oligomerization domain with an exogenous one, and assay the effects during Drosophila development. We will use a combination of chromatin immunoprecipi tation and sequencing (ChIP-Seq) in a cell culture system to determine whether Groucho is recruited to discrete sites or if it spreads along the chromatin at target genes. We will also use ChIP-Seq to establish if recruitment of Groucho leads to chromatin modifications that may induce target gene repression.
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Grant Details
Amount Awarded | 214876 |
Applicant Surname | Jennings |
Approval Committee | Molecules, Genes and Cells Funding Committee |
Award Date | 2009-10-06T00:00:00+00:00 |
Financial Year | 2009/10 |
Grant Programme: Title | Project Grant |
Internal ID | 090021/Z/09/Z |
Lead Applicant | Dr Barbara Jennings |
Partnership Value | 214876 |
Planned Dates: End Date | 2013-08-31T00:00:00+00:00 |
Planned Dates: Start Date | 2010-03-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Greater London |