From single neuron perturbation in vivo to behaviour. (360G-Wellcome-092615_Z_10_Z)

£347,783

We focus on recording local network activity while perturbing the activity of a single neuron in anaesthetized, awake and behaving animals. Using variety of methods we will maximise the precision, duration and spatiotemporal resolution of the stimulations and the recordings. Theoretical tools will be used to analyse and interpret these measurements. We will use whole cell recordings in vivo to trigger spikes in a cortical neuron and simultaneously record, using tetrodes, single unit activity fro m neighboring neurons. In awake animals we will use a complementary approach for stimulation, electroporating a single neuron with plasmids encoding for light-sensitive ion channels (e.g. ChR2) and photostimulation to trigger spikes. We will quantify how a single extra-spike increases the probability of neurons in the local network to spike. In order to identify the individual cells in the population that participate in the network activity we will combine these methods with in vivo two-photon c alcium imaging of population activity. Finally we will train mice on a whisker positioning discrimination task, and combine all these methods to establish a causal relationship between the participation of a neuron in the task and the effect of perturbing its activity on the performance of the mouse in the task.

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Grant Details

Amount Awarded 347783
Applicant Surname Hausser
Approval Committee Molecular and Cellular Neuroscience Funding Committee
Award Date 2010-07-01T00:00:00+00:00
Financial Year 2009/10
Grant Programme: Title Project Grant
Internal ID 092615/Z/10/Z
Lead Applicant Prof Michael Hausser
Other Applicant(s) Dr Michael London
Partnership Value 347783
Planned Dates: End Date 2014-08-31T00:00:00+00:00
Planned Dates: Start Date 2010-09-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London