Nanoscopy of Dynamics in the Living Cell. (360G-Wellcome-095927_B_11_Z)

PrDramatic advances in several physics-driven laboratories have established the principle of super-resolution optical imaging. Major improvements in spatial and temporal resolution are sorely needed for the technology to be useful to answer major biological questions. We propose a bold multi-disciplinary program to drive the technology forward with biological applications that demand dynamic visualization on the nanoscale in the belief that step-change technology development cannot effectively e volve without motivation and pull from those who need the applications the most. Leveraging major investments made by Yale, we have assembled a multi-disciplinary team of engineers, physicists, and cell biologists from the Gurdon Institute, the Cell Biology Department at Yale, the LMB at Cambridge, and Oxford University which over five years will develop a new generation of microscopes and probes capable of multi-color dynamic imaging deep in live cells with spatial resolution