Making memories in the mushroom bodies. (360G-Wellcome-096099_Z_11_Z)
Kenyon cell (KC) output synapses in the mushroom body (MB) lobes are the postulated sites in the fly brain where aversive olfactory memories are stored as a result of reinforcing dopamine (DA) action. However, the connectivity between KCs and the relevant DA neurons of the PPL1 cluster remains to be determined. The proposed project will examine the innervation patterns of PPL1 neurons to the MB lobes. Single KCs and single PPL1 neurons will be labelled and their synaptic contacts imaged by S TED microscopy. This will shed light on the long-standing question of whether KC output synapses are tripartite structures. In a second aim, I will analyse DA modulation of synaptic weights at KC output synapses. Stimulating KCs via a patch pipette, and DA neurons by light-activated ion channels, will allow me to monitor changes in KC synaptic weights optically, using synaptopHluorin or synaptically localised calcium probes. I will next study the metaplastic regulation of KC output synaps es, i.e. the regulation of the relevant DA neurons by the neuropeptide dNPF, which is a critical factor signalling the animal's internal state of hunger. These results will resolve mechanisms underlying the motivational control of memory retrieval.
Where is this data from?
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Grant Details
Amount Awarded | 250000 |
Applicant Surname | Owald |
Approval Committee | Sir Henry Wellcome Postdoctoral Fellowships Interview Committee |
Award Date | 2011-05-23T00:00:00+00:00 |
Financial Year | 2010/11 |
Grant Programme: Title | Sir Henry Wellcome Postdoctoral Fellowship |
Internal ID | 096099/Z/11/Z |
Lead Applicant | Dr David Owald |
Partnership Value | 250000 |
Planned Dates: End Date | 2016-10-31T00:00:00+00:00 |
Planned Dates: Start Date | 2012-11-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | South East |
Sponsor(s) | Prof Gero Miesenboeck |