Characterisation of genes regulated by S-nitrosylation of HDAC2 during radial neuron migration. (360G-Wellcome-096619_Z_11_Z)

£157,778

The generation of the laminar structure of the cerebral cortex relies on the radial migration of post mitotic neurons. This proceeds under the control of extremely precise regulatory networks requiring input from multiple extracellular signalling pathways and the co-ordinated expression of nuclear factors that result in specific programmes of gene expression. Recently, in the Riccio lab, we have shown that S-nitrosylation of HDAC2 is required for polarization and radial migration of cortical neurons during the formation of the cerebral cortex. A screen was carried out to identify genes regulated by S-nitrosylation of HDAC2 during cortical neuron migration. Druing this project, 2 candidate genes identified by this screen will be selected and their role in cortical development will be characterised. Key goals which have been set in order to achieve this include the following: 1) Verifying that these genes are upregulated in response to HDAC2 S-nitrosylation. 2) Characterisation of candidate genes expression in vivo 3) Analysis of candidate gene promoter and interaction with HDAC2 4) Characterisation of the function of the candidate genes in cortical neuron polarization and migration, which will include the generation of 2 knockout mouse lines.

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Grant Details

Amount Awarded 157778
Applicant Surname Sherlock
Approval Committee PhD Studentships
Award Date 2011-07-12T00:00:00+00:00
Financial Year 2010/11
Grant Programme: Title PhD Studentship (Basic)
Internal ID 096619/Z/11/Z
Lead Applicant Mr William Sherlock
Partnership Value 157778
Planned Dates: End Date 2015-09-30T00:00:00+00:00
Planned Dates: Start Date 2011-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London
Sponsor(s) Prof Claudio Stern