Structural analysis of unconventional kinetochore proteins in Trypanosoma brucei (360G-Wellcome-098403_Z_12_B)

£178,556

Faithful chromosome segregation is essential for the proliferation of all organisms. Although studies in popular model eukaryotes have found that macromolecular kinetochore complexes assemble onto centromeric DNA to facilitate segregation, it is not known whether this mechanism applies to all eukaryotes. To uncover fundamental principles of eukaryotic segregation machinery, I am studying kinetochore functions in Trypanosoma brucei, an evolutionary distant eukaryotic parasite. No kinetochore prot eins has been identified and thus how trypanosomes segregate their chromosomes remains a black box. In my pilot study, I carried out a localization-based screening and proteomics and identified 12 kinetochore proteins. In this proposal, I aim to identify the complete kinetochore proteomes in T. brucei. Identified kinetochore proteins will be characterized using various techniques both in vivo and in vitro to reveal the molecular mechanism of chromosome segregation in trypanosomes. By revealing w hich features are fundamental and which are species-specific, I aim to understand the design principles of kinetochores that facilitate chromosome segregation with exquisite accuracy. Understanding the mechanism of chromosome segregation in trypanosomes is also medically important to develop better treatment for these diseases.

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Grant Details

Amount Awarded 178556
Applicant Surname Akiyoshi
Approval Committee Science Enhancement Committee
Award Date 2016-06-08T00:00:00+00:00
Financial Year 2015/16
Grant Programme: Title Enhancement
Internal ID 098403/Z/12/B
Lead Applicant Dr Bungo Akiyoshi
Partnership Name Royal Society/Wellcome Trust Sir Henry Dale Fellowship
Partnership Value 178556
Planned Dates: End Date 2018-09-30T00:00:00+00:00
Planned Dates: Start Date 2016-07-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region South East