Structural analysis of unconventional kinetochore proteins in Trypanosoma brucei (360G-Wellcome-098403_Z_12_B)
Faithful chromosome segregation is essential for the proliferation of all organisms. Although studies in popular model eukaryotes have found that macromolecular kinetochore complexes assemble onto centromeric DNA to facilitate segregation, it is not known whether this mechanism applies to all eukaryotes. To uncover fundamental principles of eukaryotic segregation machinery, I am studying kinetochore functions in Trypanosoma brucei, an evolutionary distant eukaryotic parasite. No kinetochore prot eins has been identified and thus how trypanosomes segregate their chromosomes remains a black box. In my pilot study, I carried out a localization-based screening and proteomics and identified 12 kinetochore proteins. In this proposal, I aim to identify the complete kinetochore proteomes in T. brucei. Identified kinetochore proteins will be characterized using various techniques both in vivo and in vitro to reveal the molecular mechanism of chromosome segregation in trypanosomes. By revealing w hich features are fundamental and which are species-specific, I aim to understand the design principles of kinetochores that facilitate chromosome segregation with exquisite accuracy. Understanding the mechanism of chromosome segregation in trypanosomes is also medically important to develop better treatment for these diseases.
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Grant Details
Amount Awarded | 178556 |
Applicant Surname | Akiyoshi |
Approval Committee | Science Enhancement Committee |
Award Date | 2016-06-08T00:00:00+00:00 |
Financial Year | 2015/16 |
Grant Programme: Title | Enhancement |
Internal ID | 098403/Z/12/B |
Lead Applicant | Dr Bungo Akiyoshi |
Partnership Name | Royal Society/Wellcome Trust Sir Henry Dale Fellowship |
Partnership Value | 178556 |
Planned Dates: End Date | 2018-09-30T00:00:00+00:00 |
Planned Dates: Start Date | 2016-07-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | South East |