Biophysical and structural characterisation of an in vitro-assembled viral DNApackaging system (360G-Wellcome-102294_Z_13_Z)
During the assembly of herpes viruses a DNA packaging motor inserts the viral genome into procapsids. Related motors from tailed bacteriophages have proved to be excellent models for understanding this process. Thus, we propose to assemble in vitro the motor system of Escherichia coli phage HK97 for further biophysical and structural studies. Single-molecule magnetic tweezers experiments will be conducted to explore the kinetics and thermodynamics of packaging as well as the requirements for a functional system. Electron microscopy and Xray crystallography will be used to determine structures of the assembly intermediates and of individual components and sub-complexes. Ultimately, we hope to elucidate the mechanisms of viral DNA packaging so that we are better informed when studying more complicated systems such as herpes viruses.
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Grant Details
Amount Awarded | 149673 |
Applicant Surname | Fung |
Approval Committee | PhD Studentships |
Award Date | 2013-06-24T00:00:00+00:00 |
Financial Year | 2012/13 |
Grant Programme: Title | PhD Studentship (Basic) |
Internal ID | 102294/Z/13/Z |
Lead Applicant | Mr Herman Fung |
Partnership Value | 149673 |
Planned Dates: End Date | 2017-09-30T00:00:00+00:00 |
Planned Dates: Start Date | 2013-10-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Yorkshire and the Humber |
Sponsor(s) | Prof Deborah Smith |