Biophysical and structural characterisation of an in vitro-assembled viral DNApackaging system (360G-Wellcome-102294_Z_13_Z)

£149,673

During the assembly of herpes viruses a DNA packaging motor inserts the viral genome into procapsids. Related motors from tailed bacteriophages have proved to be excellent models for understanding this process. Thus, we propose to assemble in vitro the motor system of Escherichia coli phage HK97 for further biophysical and structural studies. Single-molecule magnetic tweezers experiments will be conducted to explore the kinetics and thermodynamics of packaging as well as the requirements for a functional system. Electron microscopy and Xray crystallography will be used to determine structures of the assembly intermediates and of individual components and sub-complexes. Ultimately, we hope to elucidate the mechanisms of viral DNA packaging so that we are better informed when studying more complicated systems such as herpes viruses.

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Grant Details

Amount Awarded 149673
Applicant Surname Fung
Approval Committee PhD Studentships
Award Date 2013-06-24T00:00:00+00:00
Financial Year 2012/13
Grant Programme: Title PhD Studentship (Basic)
Internal ID 102294/Z/13/Z
Lead Applicant Mr Herman Fung
Partnership Value 149673
Planned Dates: End Date 2017-09-30T00:00:00+00:00
Planned Dates: Start Date 2013-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Yorkshire and the Humber
Sponsor(s) Prof Deborah Smith