Public Engagement provision (360G-Wellcome-103930_Z_14_A)

£5,000

The aim of my research is to understand the molecular mechanisms that govern polarized secretion at the immunological synapse. We will use the CTL model with a clear trigger (TCR) and end-point (target cell death) to identify and characterize proteins that play a role in polarization and secretion as the immunological synapse is formed. Our specific aims over the next 5 years will be to (i) Map the order of events leading to secretion at the immunological synapse using rapid microscopy to compare the sequence of events leading to secretion in wild-type versus mutant CTL. (ii) Identify novel mechanisms contributing to polarised secretion in CTL by identifying genes up-regulated by Hh signaling that facilitate centrosome polarization; analyzing CTL derived from ciliary mutations that disrupt Hh signalling, gene-deletion mice from the Sanger screen and using proteomics to identify interactions with known proteins. (iii) Determine whether the lysosome itself plays a role in controlling polarization and secretion at the immunological synapse by asking whether TFEB regulates gene expression of proteins required for CTL secretion, and whether localized release of calcium from lysosomes via TPC1 and TPC2 controls any of the steps leading to polarized secretion from CTL.

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Grant Details

Amount Awarded 5000
Applicant Surname Griffiths
Approval Committee Science Interview Panel
Award Date 2014-04-01T00:00:00+00:00
Financial Year 2013/14
Grant Programme: Title Provision for Public Engagement
Internal ID 103930/Z/14/A
Lead Applicant Prof Gillian Griffiths
Partnership Value 5000
Planned Dates: End Date 2019-08-31T00:00:00+00:00
Planned Dates: Start Date 2014-09-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England
Sponsor(s) Prof Patrick Maxwell