In vivo microscopy system to probe microbiome link to inflammation (360G-Wellcome-202911_Z_16_Z)

£284,088

The microbiome has been shown to impact immune responses in the gut and throughout the body. While there are many multiphoton microscopes available in the UK and at the University of Oxford, none of these systems combine the features necessary to image fluorescent reporters in tissues under varying conditions of inflammation or are juxtaposed to a state of the art germ free animal facility. Wellcome Trust funded investigators at the University of Oxford request a microscope specialized for in vivo imaging of inflammatory processes in multiple impacted tissues. The special features of the system will be large field of view, high scan rate and innovative use of a spectral detector array and dual laser multiphoton excitation. The system will be situated in the Kennedy Trust of Rheumatology animal facility, which will include both specific pathogen free and germ-free/gnotobiotic mice. We request a Zeiss 880 laser scanning microscope with non-descanned and spectral GaAsP detectors with a set of conventional lasers to combine the complementary strengths of multiphoton and single photon excitation with conventional non-descanned detectors and highly sensitive spectral detectors that can be used in different combinations to enable flexible imaging approaches for challenging inflamed tissues.

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Grant Details

Amount Awarded 284088
Applicant Surname Dustin
Approval Committee Multi-User Equipment and Technology Development Committee
Award Date 2016-06-16T00:00:00+00:00
Financial Year 2015/16
Grant Programme: Title Multi-User Equipment Grant
Internal ID 202911/Z/16/Z
Lead Applicant Prof Michael Dustin
Other Applicant(s) Dr Audrey Gerard, Dr Francis Szele, Dr Tal Arnon, Prof Alison Simmons, Prof David Bennett, Prof Fiona Powrie, Prof Graham Ogg, Prof Kevin Maloy, Prof Paul Klenerman
Partnership Value 284088
Planned Dates: End Date 2017-06-28T00:00:00+00:00
Planned Dates: Start Date 2016-06-28T00:00:00+00:00
Recipient Org: Country United Kingdom
Region South East