Assessing the effect of the Xrn1 exoribonuclease on global and specific rates of protein synthesis (360G-Wellcome-206965_Z_17_Z)

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Gene expression is a complex process requiring the coordination of transcription, transcript turnover, translation and proteostasis. When coordinated, these processes can be adapted to allow cells to respond to changes in growth rate and during differentiation. How these processes are coordinated remains a long-standing question. The exoribonuclease Xrn1 is proposed to coordinate transcription and translation via control of steady-state RNA levels and may be part of the mechanism. In this proposal we intend to test one aspect of the proposed model: that Xrn1 regulates steady-state RNA levels and this in turn affects steady-state protein levels. We predict that rates of protein synthesis will change as steady-state RNA levels change and this relationship will be disrupted in cells lacking Xrn1. To assess rates of protein synthesis, we will pulse label cells with puromycin and use a specific antibody to assess the degree of global and specific protein puromycylation in the presence and absence of Xrn1. By assessing rates of protein synthesis for specific proteins we will be able to relate this to levels of protein, transcripts and transcription in the WT and xrn1Delta strains, obtain preliminary data to validate our hypothesis and if encouraging to support a larger scale analysis.

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Grant Details

Amount Awarded 0
Applicant Surname Roberts
Approval Committee Internal Decision Panel
Award Date 2017-04-27T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Vacation Scholarships
Internal ID 206965/Z/17/Z
Lead Applicant Mr Emile Roberts
Partnership Value 0
Planned Dates: End Date 2017-09-02T00:00:00+00:00
Planned Dates: Start Date 2017-07-03T00:00:00+00:00
Recipient Org: Country United Kingdom
Region South East