Can factors secreted by MUC1-ST educated monocytes influence breast cancer stem cell growth? (360G-Wellcome-207173_Z_17_Z)

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Aberrant glycosylation of glycoproteins expressed by cancers can lead to tumour:immune cell interactions that are cancer specific. One such interaction is the binding of a tumour-associated glycoform of the mucin, MUC1-ST, to the sialic binding lectin, Siglec-9, expressed by monocytes and macrophages. The engagement of Siglec-9 by MUC1-ST, leads to the secretion of factors by monocytes associated with tumour-progression. Interestingly, one such factor that has been identified is growth hormone and the growth hormone receptor has been found to be associated with normal mammary stem cells. To investigate if growth hormone or other factors secreted by MUC1-ST educated monocytes can influence breast cancer stem cells we will examine the extent to which breast cancer cells lines show cancer stem cell properties by: 1. Determining the expression of the breast stem cell markers (CD44 and CD24) using flow cytometry, in the presence or absence of media from MUC1-ST educated monocytes. 2. Looking at mammosphere growth in the same medium, where we will also take into account the production of GH by blocking the GHR using specific blocking antibodies.

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Grant Details

Amount Awarded 0
Applicant Surname Samelis
Approval Committee Internal Decision Panel
Award Date 2017-04-27T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Vacation Scholarships
Internal ID 207173/Z/17/Z
Lead Applicant Mr Vasileios Samelis
Partnership Value 0
Planned Dates: End Date 2017-08-11T00:00:00+00:00
Planned Dates: Start Date 2017-06-12T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London