The chemical biology and function of natural modified DNA bases in genomes (360G-Wellcome-209441_Z_17_Z)
I aim to elucidate the function of natural, chemically-modified DNA bases in the genomes of model organisms, using chemical biology and physical science approaches on genomic DNA. Modified bases are of fundamental importance to transcriptional programming and cell identity during and after development. The role of the cytosine derivative 5-formylcytosine and its influence on nucleosome formation, active enhancers, transcription and cell identity will be one area of focus to build mechanistic understanding, following on from hypotheses derived from our prior work. There will also be an investigation of 5-carboxycytosine and 5-hydroxymethyluridine and their potential links with transcription regulation. For other modified bases, such as N6-methyladenine, we will develop and use new chemical mapping/sequencing methods to elucidate their function in mammalian systems. The programme will include a systematic discovery of other natural DNA base modifications, building on and augmenting chemical methodologies I have developed to discover and profile modified bases in RNA. The function of newly identified base modifications will be investigated during the programme. The insights provided from these fundamental studies may have far-reaching consequences for normal biology and disease states. Keywords: chemical biology, nucleic acids, DNA, modified bases, epigenetics, sequencing
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.
Grant Details
Amount Awarded | 2198391 |
Applicant Surname | Balasubramanian |
Approval Committee | Science Interview Panel |
Award Date | 2017-11-28T00:00:00+00:00 |
Financial Year | 2017/18 |
Grant Programme: Title | Investigator Award in Science |
Internal ID | 209441/Z/17/Z |
Lead Applicant | Prof Sir Shankar Balasubramanian |
Partnership Value | 2198391 |
Planned Dates: End Date | 2024-12-01T00:00:00+00:00 |
Planned Dates: Start Date | 2018-12-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | East of England |