Congenic cell bank for pigmentary mutations (360G-Wellcome-078327_Z_05_Z)

£491,972

The Wellcome Trust Functional Genomics Cell Bank holds a large collection of mammalian cell lines, especially mouse melanocyte and melanoblast lines carrying specific pigmentary mutations, and provides these to researchers as a service, together with advice on melanocyte culture. We request funds to continue this service for a second quinquennium. We will establish additional lines of mutant mouse and human melanocytes known to be of current research interest. We will also use cells from the collection ourselves, in research on two main areas. One is the mechanism of cell determination from pluripotent precursors, using the melanocyte lineage as a model. We will use our pluripotent neural crest-like cell lines, and existing and new melanocyte and melanoblast lines deficient in key transcription factors, especially the putative master controller Mitf. We will examine the dynamics of changes after induction of (exogenous) Mitf expression, and effects of different levels of the factor. Global mRNA and protein analyses by microarrays and DIGE will be followed by focussing on changes in candidate regulators, then building and testing of model(s) integrating available knowledge. The other area is that of the regulators and effectors involved in the synthesis of pheomelanin (red/yellow pigment), still very poorly understood.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 491972
Applicant Surname Bennett
Approval Committee Molecules, Genes and Cells Funding Committee
Award Date 2005-10-17T00:00:00+00:00
Financial Year 2005/06
Grant Programme: Title Biomedical Resources Grant
Internal ID 078327/Z/05/Z
Lead Applicant Prof Dorothy Bennett
Other Applicant(s) Dr Elena Sviderskaya
Partnership Value 491972
Planned Dates: End Date 2011-05-31T00:00:00+00:00
Planned Dates: Start Date 2006-06-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London