Congenic cell bank for pigmentary mutations (360G-Wellcome-078327_Z_05_Z)
The Wellcome Trust Functional Genomics Cell Bank holds a large collection of mammalian cell lines, especially mouse melanocyte and melanoblast lines carrying specific pigmentary mutations, and provides these to researchers as a service, together with advice on melanocyte culture. We request funds to continue this service for a second quinquennium. We will establish additional lines of mutant mouse and human melanocytes known to be of current research interest. We will also use cells from the collection ourselves, in research on two main areas. One is the mechanism of cell determination from pluripotent precursors, using the melanocyte lineage as a model. We will use our pluripotent neural crest-like cell lines, and existing and new melanocyte and melanoblast lines deficient in key transcription factors, especially the putative master controller Mitf. We will examine the dynamics of changes after induction of (exogenous) Mitf expression, and effects of different levels of the factor. Global mRNA and protein analyses by microarrays and DIGE will be followed by focussing on changes in candidate regulators, then building and testing of model(s) integrating available knowledge. The other area is that of the regulators and effectors involved in the synthesis of pheomelanin (red/yellow pigment), still very poorly understood.
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Grant Details
Amount Awarded | 491972 |
Applicant Surname | Bennett |
Approval Committee | Molecules, Genes and Cells Funding Committee |
Award Date | 2005-10-17T00:00:00+00:00 |
Financial Year | 2005/06 |
Grant Programme: Title | Biomedical Resources Grant |
Internal ID | 078327/Z/05/Z |
Lead Applicant | Prof Dorothy Bennett |
Other Applicant(s) | Dr Elena Sviderskaya |
Partnership Value | 491972 |
Planned Dates: End Date | 2011-05-31T00:00:00+00:00 |
Planned Dates: Start Date | 2006-06-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Greater London |