Targeting and regulation of kinesin-1. (360G-Wellcome-078825_Z_05_Z)

£262,353

Although it has been proposed that different kinesin-1 isoforms are targeted to particular cargoes, functional evidence for this hypothesis has been lacking. Using assays in which membrane movement along microtubules is reconstituted in vitro, we have evidence that kinesin light chain (KLC) 1B is involved in rough endoplasmic reticulum (RER) movement, while KLC1D is required for movement of vesicles in a Golgi fraction. So far, all of the proteins identified as kinesin-1 interactors will bind to all forms of kinesin-1. Our first goal, therefore, is to identify and characterise proteins which interact with KLC1B but not KLC1D, and vice versa. Kinesin-1, like other motors, is very likely to be tightly regulated so that the right cargo moves at the right time. Currently, however, we know almost nothing about the way in which kinesin-1 is regulated in non-neuronal cells. Our recent work has revealed a novel role for dynactin in activating kinesin-1-driven RER and Golgi vesicle movement. Dy nactin was first identified as a regulator of cytoplasmic dynein, a microtubule motor which moves in the opposite direction along microtubules compared to kinesin-1. Our second goal is investigate how dynactin regulates kinesin-1 activity.

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Grant Details

Amount Awarded 262353
Applicant Surname Allan
Approval Committee Molecules, Genes and Cells Funding Committee
Award Date 2006-02-21T00:00:00+00:00
Financial Year 2005/06
Grant Programme: Title Project Grant
Internal ID 078825/Z/05/Z
Lead Applicant Prof Victoria Allan
Partnership Value 262353
Planned Dates: End Date 2009-09-30T00:00:00+00:00
Planned Dates: Start Date 2006-03-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region North West