Genetic manipulation of Chlamydia trachomatis. (360G-Wellcome-080348_Z_06_Z)

£284,679

The field of chlamydia research has been hampered by the inability to take a direct genetic approach to study the function and regulation of genes. The development of a system by which modified genes can be introduced will provide a significant boost to studies on this important human pathogen. Preliminary data generated in collaboration with Dr. Kahane and Prof Friedmann, presented in this application, provides 'proof of principle' that this much sought after technological goal has been reached. However, the protocol developed is time consuming, requires an experienced chlamydiologist and high level cell culture skills.Using this successful approach as a starting point we now plan to develop an efficient gene transfer system for Chlamydia trachomatis. Our key goal is to improve the protocol making it possible to establish this technology as a routine procedure for the study of chlamydial molecular biology. This will be achieved by improving the efficiency of the method, producing improved vectors for selection of transformants and using plasmid free chlamydial host strains for transformation studies.

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Grant Details

Amount Awarded 284679
Applicant Surname Clarke
Approval Committee Immunology and Infectious Disease Funding Committee
Award Date 2006-10-19T00:00:00+00:00
Financial Year 2006/07
Grant Programme: Title Project Grant
Internal ID 080348/Z/06/Z
Lead Applicant Prof Ian Clarke
Other Applicant(s) Dr Paul Lambden
Partnership Value 284679
Planned Dates: End Date 2010-06-30T00:00:00+00:00
Planned Dates: Start Date 2007-04-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region South East