Genetic manipulation of Chlamydia trachomatis. (360G-Wellcome-080348_Z_06_Z)
The field of chlamydia research has been hampered by the inability to take a direct genetic approach to study the function and regulation of genes. The development of a system by which modified genes can be introduced will provide a significant boost to studies on this important human pathogen. Preliminary data generated in collaboration with Dr. Kahane and Prof Friedmann, presented in this application, provides 'proof of principle' that this much sought after technological goal has been reached. However, the protocol developed is time consuming, requires an experienced chlamydiologist and high level cell culture skills.Using this successful approach as a starting point we now plan to develop an efficient gene transfer system for Chlamydia trachomatis. Our key goal is to improve the protocol making it possible to establish this technology as a routine procedure for the study of chlamydial molecular biology. This will be achieved by improving the efficiency of the method, producing improved vectors for selection of transformants and using plasmid free chlamydial host strains for transformation studies.
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Grant Details
Amount Awarded | 284679 |
Applicant Surname | Clarke |
Approval Committee | Immunology and Infectious Disease Funding Committee |
Award Date | 2006-10-19T00:00:00+00:00 |
Financial Year | 2006/07 |
Grant Programme: Title | Project Grant |
Internal ID | 080348/Z/06/Z |
Lead Applicant | Prof Ian Clarke |
Other Applicant(s) | Dr Paul Lambden |
Partnership Value | 284679 |
Planned Dates: End Date | 2010-06-30T00:00:00+00:00 |
Planned Dates: Start Date | 2007-04-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | South East |