Processes of human meiotic recombination. (360G-Wellcome-081227_Z_06_Z)
We are using single molecule methods to access recombinant DNA molecules directly in sperm, to characterise basic features of human meiotic recombination such as clustering of exchanges into hotspots, identification offactors that control hotspot activity and the role of hotspots in driving genome instability. However, these studies have sampled very few hotspots in the human genome and the potentially most informative sites for investigating recombination have yet to be characterised. Also, sperm analysis gives information on resolution sites in meiotic recombination, but not on recombination intermediates. This project will therefore identify the most active hotspots in the human genome and will use these as a resource not only to explore hotspot properties but also to directly access intermediates in therecombination process. Targets include the recovery of resection tracts extending from recombination-initiating double strand DNA breaks in testis andthe isolation of later intermediates in the recombination pathway. This will give direct information on DNA processing during human meiotic recombination, including the location of initiation sites, resection tract lengths and distributions, and the location of Holliday junctions. We will also use these and other hotspots to test directly whether recombination is base mutagenic.
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.
Grant Details
Amount Awarded | 177024 |
Applicant Surname | Jeffreys |
Approval Committee | Molecules, Genes and Cells Funding Committee |
Award Date | 2006-11-01T00:00:00+00:00 |
Financial Year | 2006/07 |
Grant Programme: Title | Project Grant |
Internal ID | 081227/Z/06/Z |
Lead Applicant | Prof Sir Alec Jeffreys |
Partnership Value | 177024 |
Planned Dates: End Date | 2011-07-31T00:00:00+00:00 |
Planned Dates: Start Date | 2008-01-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | East Midlands |