Processes of human meiotic recombination. (360G-Wellcome-081227_Z_06_Z)

£177,024

We are using single molecule methods to access recombinant DNA molecules directly in sperm, to characterise basic features of human meiotic recombination such as clustering of exchanges into hotspots, identification offactors that control hotspot activity and the role of hotspots in driving genome instability. However, these studies have sampled very few hotspots in the human genome and the potentially most informative sites for investigating recombination have yet to be characterised. Also, sperm analysis gives information on resolution sites in meiotic recombination, but not on recombination intermediates. This project will therefore identify the most active hotspots in the human genome and will use these as a resource not only to explore hotspot properties but also to directly access intermediates in therecombination process. Targets include the recovery of resection tracts extending from recombination-initiating double strand DNA breaks in testis andthe isolation of later intermediates in the recombination pathway. This will give direct information on DNA processing during human meiotic recombination, including the location of initiation sites, resection tract lengths and distributions, and the location of Holliday junctions. We will also use these and other hotspots to test directly whether recombination is base mutagenic.

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Grant Details

Amount Awarded 177024
Applicant Surname Jeffreys
Approval Committee Molecules, Genes and Cells Funding Committee
Award Date 2006-11-01T00:00:00+00:00
Financial Year 2006/07
Grant Programme: Title Project Grant
Internal ID 081227/Z/06/Z
Lead Applicant Prof Sir Alec Jeffreys
Partnership Value 177024
Planned Dates: End Date 2011-07-31T00:00:00+00:00
Planned Dates: Start Date 2008-01-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East Midlands