Structural biology of bacterial type IV secretion systems. (360G-Wellcome-082227_Z_07_Z)

£1,655,214

Type IV secretion machines are composed of at least 12 proteins termed VirB1-11 and VirD4. In the previous grant period, we have solved the structures of six of the VirB proteins or fragments thereof (VirB5, 7, 8, 9, 10, and 11). We have initiated a programme aiming at providing the structures of complexes of VirB proteins with each other. One of these complex structures has been solved. A massive cloning effort has led to the identification and purification of 3 more complexes. For the next gra nt period, we will focus on protein complexes. We will continue our cloning co-expression effort in view of identifying additional binary, ternary, and quaternary complexes. We will image existing and future complexes using cryo-electron microscopy, NMR, and x-ray crystallography. We will also attempt to purify the entire type IV secretion machine with the long-term aim to produce high-resolution cryo-EM maps into which individual single or complex structures can be docked. Our ultimate aim is t o provide a view of the type IV secretion machine to understand how it works.

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Grant Details

Amount Awarded 1655214
Applicant Surname Waksman
Approval Committee Immunology and Infectious Disease Funding Committee
Award Date 2007-04-24T00:00:00+00:00
Financial Year 2006/07
Grant Programme: Title Programme Grant
Internal ID 082227/Z/07/Z
Lead Applicant Prof Gabriel Waksman
Partnership Value 1655214
Planned Dates: End Date 2013-03-31T00:00:00+00:00
Planned Dates: Start Date 2008-04-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London