Structural biology of bacterial type IV secretion systems. (360G-Wellcome-082227_Z_07_Z)
Type IV secretion machines are composed of at least 12 proteins termed VirB1-11 and VirD4. In the previous grant period, we have solved the structures of six of the VirB proteins or fragments thereof (VirB5, 7, 8, 9, 10, and 11). We have initiated a programme aiming at providing the structures of complexes of VirB proteins with each other. One of these complex structures has been solved. A massive cloning effort has led to the identification and purification of 3 more complexes. For the next gra nt period, we will focus on protein complexes. We will continue our cloning co-expression effort in view of identifying additional binary, ternary, and quaternary complexes. We will image existing and future complexes using cryo-electron microscopy, NMR, and x-ray crystallography. We will also attempt to purify the entire type IV secretion machine with the long-term aim to produce high-resolution cryo-EM maps into which individual single or complex structures can be docked. Our ultimate aim is t o provide a view of the type IV secretion machine to understand how it works.
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Grant Details
Amount Awarded | 1655214 |
Applicant Surname | Waksman |
Approval Committee | Immunology and Infectious Disease Funding Committee |
Award Date | 2007-04-24T00:00:00+00:00 |
Financial Year | 2006/07 |
Grant Programme: Title | Programme Grant |
Internal ID | 082227/Z/07/Z |
Lead Applicant | Prof Gabriel Waksman |
Partnership Value | 1655214 |
Planned Dates: End Date | 2013-03-31T00:00:00+00:00 |
Planned Dates: Start Date | 2008-04-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Greater London |