tRNA retrograde transport and HIV-1 nuclear trafficking: a surprising connection. (360G-Wellcome-083810_Z_07_Z)
We have recently discovered that cytoplasmic tRNAs can be transported back into the nucleus in human cells, a process called retrograde tRNA transport, and that this process is exploited by HIV-1 to infect non dividing cells. We hypothesize that specific cellular factors drive nuclear import of tRNAs in human cells and that HIV-1 nuclear import is also promoted by the same factors. We aim to investigate this by: 1) Isolating the cellular factors driving tRNA nuclear import by chromatographic an d genetic approaches. 2) Disrupting their function by stable knock down or generation of dominant-negative mutants and examine the impact on HIV-1 infection in dividing and non-dividing cells. 3) Mapping the viral determinants for incorporation of and interaction with tRNAs with nuclear import activity by the generation of HIV-1 and murine leukaemia virus (MLV) mutants.
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Grant Details
Amount Awarded | 367002 |
Applicant Surname | Fassati |
Approval Committee | Immunology and Infectious Disease Funding Committee |
Award Date | 2007-10-24T00:00:00+00:00 |
Financial Year | 2007/08 |
Grant Programme: Title | Project Grant |
Internal ID | 083810/Z/07/Z |
Lead Applicant | Prof Ariberto Fassati |
Partnership Value | 367002 |
Planned Dates: End Date | 2011-05-31T00:00:00+00:00 |
Planned Dates: Start Date | 2007-12-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Greater London |