A genome-wide approach to the characterisation of human and murine DNA methylation. (360G-Wellcome-084358_B_07_Z)
The proposed project is designed to assay methylation mutants in mouse and humanusing genomic microarray technology. Offspring of mice lacking DNA methyltransferase 3L (Dnmt3L) have no maternal methylation marks at imprinted loci. Genomic array technology will be used to compare mice lacking maternally derived Dnmt3L with wildtype, to identify novel regions of differential methylation and associated imprinted genes. A subset of human patients with transient neonatal diabetes (TNDM) lack maternal methylation marks at a number of imprinted loci. Furthermore, about 30% of patients with SRS show reduced methylation at the H19 DMR. DNA from these patients, like in TNDM, could also be hypomethylated at other loci, providing a rationale for their investigation. We will use methylation-specific array technology to compare a hypomethylated SRS and TNDM samples with control individuals. We will validate regions of novel differential methylation by bisulphite sequencing analysis and confirm novel imprinted genes using allele-specific assays.
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.
Grant Details
| Amount Awarded | 107120 |
| Applicant Surname | Moore |
| Approval Committee | Molecules, Genes and Cells Funding Committee |
| Award Date | 2008-02-20T00:00:00+00:00 |
| Financial Year | 2007/08 |
| Grant Programme: Title | Project Grant |
| Internal ID | 084358/B/07/Z |
| Lead Applicant | Prof Gudrun Moore |
| Other Applicant(s) | Prof Rebecca Oakey |
| Partnership Value | 107120 |
| Planned Dates: End Date | 2012-02-22T00:00:00+00:00 |
| Planned Dates: Start Date | 2009-02-23T00:00:00+00:00 |
| Recipient Org: Country | United Kingdom |
| Region | Greater London |