The role of the ovary-specific cap-binding protein in regulation of translation in early development . (360G-Wellcome-084885_Z_08_Z)
In early development, during the period from the meiotic maturation of oocytes to eggs through to onset of zygotic transcription in the fertilised embryo, the principal means of gene regulation is translational control of the masked maternal mRNA. The cytoplasmic polyadenylation element binding protein (CPEB) is a critical regulator of translation in early development, in organisms ranging from clam to man. CPEB binds CPE elements in the 3' untranslated regions of specific mRNAs, and controls t heir expression at the level of translational repression in oocytes, and by cytoplasmic polyadenylation and translational activation in eggs. In Xenopus oocytes, CPEB is present in a large RNP complex with several highly conserved RNA-binding partners, including Xp54 RNA helicase, Pat1, and RAP55, and inhibits cap-dependent protein synthesis using a novel pairing of the eIF4E-binding protein 4E-T(ransporter) and eIF4E1b, a close homologue of the canonical cap-binding protein. Our aims are to: I. Investigate eIF4E1b interactions with the m7GpppG cap II. Investigate eIF4E1b interactions with 4E-T and eIF4G III Identify mRNAs regulated by eIF4E1b IV. Assess the function of eIF4E1b in translational control V. Investigate how the interaction between eIF4E1b and 4E-T change during meiotic maturation when translation is activated
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Grant Details
Amount Awarded | 277749 |
Applicant Surname | Standart |
Approval Committee | Molecules, Genes and Cells Funding Committee |
Award Date | 2008-04-30T00:00:00+00:00 |
Financial Year | 2007/08 |
Grant Programme: Title | Project funding: Inactive scheme |
Internal ID | 084885/Z/08/Z |
Lead Applicant | Prof Nancy Standart |
Other Applicant(s) | Prof Edward Darzynkiewicz |
Partnership Value | 277749 |
Planned Dates: End Date | 2012-02-29T00:00:00+00:00 |
Planned Dates: Start Date | 2008-09-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | East of England |