The role of the ovary-specific cap-binding protein in regulation of translation in early development . (360G-Wellcome-084885_Z_08_Z)

£277,749

In early development, during the period from the meiotic maturation of oocytes to eggs through to onset of zygotic transcription in the fertilised embryo, the principal means of gene regulation is translational control of the masked maternal mRNA. The cytoplasmic polyadenylation element binding protein (CPEB) is a critical regulator of translation in early development, in organisms ranging from clam to man. CPEB binds CPE elements in the 3' untranslated regions of specific mRNAs, and controls t heir expression at the level of translational repression in oocytes, and by cytoplasmic polyadenylation and translational activation in eggs. In Xenopus oocytes, CPEB is present in a large RNP complex with several highly conserved RNA-binding partners, including Xp54 RNA helicase, Pat1, and RAP55, and inhibits cap-dependent protein synthesis using a novel pairing of the eIF4E-binding protein 4E-T(ransporter) and eIF4E1b, a close homologue of the canonical cap-binding protein. Our aims are to: I. Investigate eIF4E1b interactions with the m7GpppG cap II. Investigate eIF4E1b interactions with 4E-T and eIF4G III Identify mRNAs regulated by eIF4E1b IV. Assess the function of eIF4E1b in translational control V. Investigate how the interaction between eIF4E1b and 4E-T change during meiotic maturation when translation is activated

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Grant Details

Amount Awarded 277749
Applicant Surname Standart
Approval Committee Molecules, Genes and Cells Funding Committee
Award Date 2008-04-30T00:00:00+00:00
Financial Year 2007/08
Grant Programme: Title Project funding: Inactive scheme
Internal ID 084885/Z/08/Z
Lead Applicant Prof Nancy Standart
Other Applicant(s) Prof Edward Darzynkiewicz
Partnership Value 277749
Planned Dates: End Date 2012-02-29T00:00:00+00:00
Planned Dates: Start Date 2008-09-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England