Control of antigen processing by Fc receptors. (360G-Wellcome-084953_Z_08_Z)

£1,572,010

My proposal seeks to understand how Fcgamma receptors (FcgRs) control antigen processing by dendritic cells and macrophages. Since FcgR subtypes are variably expressed, have different binding affinities for different IgG subclasses and lead to fundamentally distinct patterns of intracellular trafficking and processing, it is not understood how, for a given repertoire of expressed FcgR, a specific antigen will be processed and what programme of effector functions will be triggered. My first key g oal will be to systematically define functional outcomes (binding and internalisation, antigen degradation and presentation) for ligand-receptor combinations using a receptor expression library in U937 cells expressing 90 different patterns of receptor subtype and disease-associated polymorphisms. Findings will then be confirmed using primary cells from healthy volunteers. I will then define the critical signal transduction events responsible for determining immunogenic and tolerogenic antigen p rocessing focussing on 1) the effect of receptor membrane sub-localisation on receptor function and cellular behaviour and 2) the role of macro-autophagy in modulating or possibly mediating FcgR-triggered intracellular antigen trafficking.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 1572010
Applicant Surname Floto
Approval Committee Clinical Interview Committee
Award Date 2008-06-05T00:00:00+00:00
Financial Year 2007/08
Grant Programme: Title Senior Research Fellowship Clinical
Internal ID 084953/Z/08/Z
Lead Applicant Prof Andres Floto
Partnership Value 1572010
Planned Dates: End Date 2014-11-30T00:00:00+00:00
Planned Dates: Start Date 2008-12-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England
Sponsor(s) Prof David Rubinsztein, Prof Paul Lehner