Molecular and cellular mechanisms of cell sorting at compartmental boundaries. (360G-Wellcome-085971_Z_08_Z)

£196,841

The molecular and cellular mechanisms underlying cell sorting during compartment formation are still not well understood. We propose to investigate this question in the early Drosophila embryo. This model has the advantage that cell sorting at compartment boundaries can be analysed in real time and with single-cell resolution. The key goals for this proposal are: 1) To develop methods to image and analyse cell behaviours as well as cytoskeleton activity in the vicinity of anterior/posterior ( A/P) boundaries in embryos. 2) We have found that Myosin II forms a cable at the A/P boundary, and thus we will investigate the function of Myosin II in cell sorting. In particular, we will use a conditional protein inactivation technique (CALI) to specifically inhibit Myosin II in this boundary cable. 3) We will conduct a deletion screen to identify novel genes required for cell sorting, and will investigate the relationship between these genes and Myosin II cabling. Our main goal for is to identify molecules, Myosin II, adhesion molecules or others, which are required for cell sorting at A/P embryonic boundaries in Drosophila. This will bring advances to boundary formation, but also further our knowledge of how the cytoskeleton controls cell behaviours in vivo.

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Grant Details

Amount Awarded 196841
Applicant Surname Sanson
Approval Committee Molecules, Genes and Cells Funding Committee
Award Date 2008-07-10T00:00:00+00:00
Financial Year 2007/08
Grant Programme: Title Project Grant
Internal ID 085971/Z/08/Z
Lead Applicant Dr Bénédicte Sanson
Partnership Value 196841
Planned Dates: End Date 2012-12-31T00:00:00+00:00
Planned Dates: Start Date 2009-01-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England