Molecular and cellular mechanisms of cell sorting at compartmental boundaries. (360G-Wellcome-085971_Z_08_Z)
The molecular and cellular mechanisms underlying cell sorting during compartment formation are still not well understood. We propose to investigate this question in the early Drosophila embryo. This model has the advantage that cell sorting at compartment boundaries can be analysed in real time and with single-cell resolution. The key goals for this proposal are: 1) To develop methods to image and analyse cell behaviours as well as cytoskeleton activity in the vicinity of anterior/posterior ( A/P) boundaries in embryos. 2) We have found that Myosin II forms a cable at the A/P boundary, and thus we will investigate the function of Myosin II in cell sorting. In particular, we will use a conditional protein inactivation technique (CALI) to specifically inhibit Myosin II in this boundary cable. 3) We will conduct a deletion screen to identify novel genes required for cell sorting, and will investigate the relationship between these genes and Myosin II cabling. Our main goal for is to identify molecules, Myosin II, adhesion molecules or others, which are required for cell sorting at A/P embryonic boundaries in Drosophila. This will bring advances to boundary formation, but also further our knowledge of how the cytoskeleton controls cell behaviours in vivo.
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.
Grant Details
Amount Awarded | 196841 |
Applicant Surname | Sanson |
Approval Committee | Molecules, Genes and Cells Funding Committee |
Award Date | 2008-07-10T00:00:00+00:00 |
Financial Year | 2007/08 |
Grant Programme: Title | Project Grant |
Internal ID | 085971/Z/08/Z |
Lead Applicant | Dr Bénédicte Sanson |
Partnership Value | 196841 |
Planned Dates: End Date | 2012-12-31T00:00:00+00:00 |
Planned Dates: Start Date | 2009-01-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | East of England |