Replication of Noroviruses: determinants of tropism. (360G-Wellcome-086112_Z_08_Z)
Human noroviruses are amongst the few remaining non-cultivable human viruses. Numerous attempts in many laboratories over the past two decades have failed to establish a routine cell culture system for the propagation of these viruses. The aims of this programme are to determine the molecular basis of tissue tropism and elucidate the interactions between viral and host cell proteins. Initially we will use the cultivable murine norovirus to investigate the role of the unique caliciviral N-term protein and we also wish to focus on the viral 3C-like protease and its role in the replication process through interactions with viral proteins, host proteins and the viral RNA genome itself. We will use our recently published reverse genetic system for the recovery of modified noroviruses from cloned cDNA. To understand the specific requirement for the replication of human noroviruses we propose to construct chimaeric viruses by replacement of sections of MNV proteins with the human equival ent. Therefore, the major aims of the programme are: 1). To determine key intracellular factors that determine norovirus tropism. 2). To investigate the intracellular role of the N-term protein 3). To investigate the role of the 3C-like protease in norovirus replication.
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Grant Details
Amount Awarded | 473899 |
Applicant Surname | Clarke |
Approval Committee | Immunology and Infectious Disease Funding Committee |
Award Date | 2008-10-02T00:00:00+00:00 |
Financial Year | 2008/09 |
Grant Programme: Title | Project Grant |
Internal ID | 086112/Z/08/Z |
Lead Applicant | Prof Ian Clarke |
Other Applicant(s) | Dr Paul Lambden |
Partnership Value | 473899 |
Planned Dates: End Date | 2013-05-31T00:00:00+00:00 |
Planned Dates: Start Date | 2009-06-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | South East |