Replication of Noroviruses: determinants of tropism. (360G-Wellcome-086112_Z_08_Z)

£473,899

Human noroviruses are amongst the few remaining non-cultivable human viruses. Numerous attempts in many laboratories over the past two decades have failed to establish a routine cell culture system for the propagation of these viruses. The aims of this programme are to determine the molecular basis of tissue tropism and elucidate the interactions between viral and host cell proteins. Initially we will use the cultivable murine norovirus to investigate the role of the unique caliciviral N-term protein and we also wish to focus on the viral 3C-like protease and its role in the replication process through interactions with viral proteins, host proteins and the viral RNA genome itself. We will use our recently published reverse genetic system for the recovery of modified noroviruses from cloned cDNA. To understand the specific requirement for the replication of human noroviruses we propose to construct chimaeric viruses by replacement of sections of MNV proteins with the human equival ent. Therefore, the major aims of the programme are: 1). To determine key intracellular factors that determine norovirus tropism. 2). To investigate the intracellular role of the N-term protein 3). To investigate the role of the 3C-like protease in norovirus replication.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 473899
Applicant Surname Clarke
Approval Committee Immunology and Infectious Disease Funding Committee
Award Date 2008-10-02T00:00:00+00:00
Financial Year 2008/09
Grant Programme: Title Project Grant
Internal ID 086112/Z/08/Z
Lead Applicant Prof Ian Clarke
Other Applicant(s) Dr Paul Lambden
Partnership Value 473899
Planned Dates: End Date 2013-05-31T00:00:00+00:00
Planned Dates: Start Date 2009-06-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region South East