Functional analysis of malarial microneme proteins essential to ookinete invasion. (360G-Wellcome-086640_Z_08_Z)

£266,380

The malarial parasite must repeatedly invade host tissues to complete its life cycle. Proteins secreted from the micronemes are essential for invasion. Microneme proteins are considered prime targets for both drug and vaccine attack. We have recently completed a proteomic analysis of the Plasmodium berghei ookinete micronemes. Here we exploit the data generated to analyse the functions of 3 partially defined, and 19 novel hypothetical-class secreted proteins. Gene deletion and fluorescent tagged mutant parasites will be generated and characterised using our widely published methodologies. Key Goal 1: Proteins critical to host invasion will be mapped to established developmental checkpoints i.e. motility, host adherence, penetration/traversal of host cells. Key Goal 2: The three micronemal proteins known to be essential to ookinete development (CTRP, PB1102 and POSH) will be examined using gene mutation methodologies to determine the structure/function relationships of the prote ins, again using established methodologies. This work has the potential to inform future drug and vaccine development.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 266380
Applicant Surname Sinden
Approval Committee Immunology and Infectious Disease Funding Committee
Award Date 2008-10-02T00:00:00+00:00
Financial Year 2008/09
Grant Programme: Title Project Grant
Internal ID 086640/Z/08/Z
Lead Applicant Prof Robert Sinden
Other Applicant(s) Dr Kalpana Lal
Partnership Value 266380
Planned Dates: End Date 2013-09-30T00:00:00+00:00
Planned Dates: Start Date 2009-01-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London