The early events in signalling by platelet ITAM and ITAM-like receptors. (360G-Wellcome-088410_Z_09_Z)
This application aims to establish (i) the very early events that underlie signalling by the three platelet ITAM receptors, GPVI, CLEC-2 and FcgammaRIIA, and (ii) the physiological and pathological roles of CLEC-2. ITAM receptors signal through sequential activation of Src and Syk tyrosine kinases, initiating a signalling cascade that leads to cell activation. The major goals of this proposal are (a) to identify the Src kinases in human and mouse platelets that mediate phosphorylation of pl atelet ITAM receptors; (b) to compare the regulation of Syk by platelet ITAM receptors; (c) to determine the molecular basis of activation of Syk by a single YxxL sequence; (d) to study the spatial and temporal nature of the protein-protein and protein-lipid interactions that underlie the very early signalling events by platelet ITAM receptors using FRET and TIRF microscopy, and (e) to determine the functional role of CLEC-2 using CLEC-2 knockout mice or mice which express a non-signalli ng mutant of CLEC-2 that retains the ability to bind to its endogenous ligand, podoplanin. These studies will focus on tissues that express CLEC-2 and podoplanin, and on a possible role of the interaction of podoplanin to CLEC-2 in cancer metastasis.
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.
Grant Details
Amount Awarded | 1246548 |
Applicant Surname | Watson |
Approval Committee | Physiological Sciences Funding Committee |
Award Date | 2009-05-07T00:00:00+00:00 |
Financial Year | 2008/09 |
Grant Programme: Title | Programme Grant |
Internal ID | 088410/Z/09/Z |
Lead Applicant | Prof Stephen Watson |
Partnership Value | 1246548 |
Planned Dates: End Date | 2016-03-31T00:00:00+00:00 |
Planned Dates: Start Date | 2009-08-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | West Midlands |