Insulator dynamics in gene regulation in Drosophila. (360G-Wellcome-089834_Z_09_Z)
Insulator elements are proposed to play key roles in the organisation of the eukaryotic genome and in gene regulation. In this project we will investigate the association between insulator proteins and the regulation of gene expression. Using ChIP-array to map the binding profiles of insulator components in specific tissues we will examine insulator protein binding to genes in different states of activity. This will identify which insulator complexes, and which specific insulator components, are constant, contributing to the fixed architecture of the genome, and which vary with gene expression state, highlighting a role in the regulation of gene expression. We will use functional assays to test these roles and we will link insulator binding to changes in chromatin configuration in different activity states. We will focus on the Ubx Hox gene and will use the 4C chromatin conformation assay to investigate the configuration of the Ubx gene both the ON and OFF states. Having both a h igh resolution map of chromatin configuration and a detailed profile of insulator protein binding in differing states of gene expression will give us an unprecedented view of the association between insulator complexes and the regulation of gene expression.
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Grant Details
Amount Awarded | 236147 |
Applicant Surname | White |
Approval Committee | Molecules, Genes and Cells Funding Committee |
Award Date | 2009-10-06T00:00:00+00:00 |
Financial Year | 2009/10 |
Grant Programme: Title | Project Grant |
Internal ID | 089834/Z/09/Z |
Lead Applicant | Dr Robert White |
Other Applicant(s) | Prof Steven Russell |
Partnership Value | 236147 |
Planned Dates: End Date | 2013-09-30T00:00:00+00:00 |
Planned Dates: Start Date | 2010-04-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | East of England |