Wellcome Trust PhD Programme for Clinicians at Imperial College London: 'Characterisation of the electrophysiological properties of cardiac myocytes derived from induced pluripotent and embryonic stem cells'. (360G-Wellcome-090790_Z_09_A)
Induced Pluripotent Stem Cells (iPSC) are created from adult somatic cells by ectopic expression of key 'stemness' transcription factors, avoiding the ethically problematic use of Embryonic Stem Cells (ESC). Autogenic iPSC also avoid technical problems associated with ESC, such as host immune rejection. Consequently iPSC derived Cardiomyocytes (iPSC-CM) may be more suitable for use in regenerative medicine and as in-vitro disease models. To ensure that iPSC-CM are safe and effective it is important to establish that they are capable of full differentiation and maturation. Furthermore their use as in-vitro disease models must be validated by comparison with human ESC derived Cardiomyocytes (ESC-CM), adult human cardiomyocytes and previously characterised animal heart preparations. As techniques to evaluate gene expression and cell morphology have significant limitations in determining the phenotype of both developing and adult cardiomyocytes characterisation of the electrophysiological properties of iPSC-CM is critical. The electrophysiological properties of singleand multi-cellular iPSC-CM preparations will be compared with ESC-CM, adult human heart tissue routinely removed during cardiac surgery, and well characterised animal heart preparations. Several techniques including multi-electrode arrays, patch clamping, voltage sensitive dyes and optical mapping techniques will be used.
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Grant Details
Amount Awarded | 13755 |
Applicant Surname | Rao |
Approval Committee | Neurosciences And Mental Health |
Award Date | 2010-06-14T00:00:00+00:00 |
Financial Year | 2009/10 |
Grant Programme: Title | PhD Training Fellowship for Clinicians |
Internal ID | 090790/Z/09/A |
Lead Applicant | Dr Christopher Rao |
Partnership Value | 13755 |
Planned Dates: End Date | 2012-10-31T00:00:00+00:00 |
Planned Dates: Start Date | 2010-06-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Greater London |
Sponsor(s) | Lord Ara Darzi, Mr Thanos Athanasiou, Prof Charles Pusey, Prof Sian Harding |