Robust workflow for sub-volume averaging of tomographic data. (360G-Wellcome-090895_Z_09_A)
Viruses and their interactions with host cells provide attractive model systems for studying macromolecular interactions. The structural design of viruses provides a remarkable example of simplicity and functionality in biological systems. Viral particles work as highly effective molecular devices in the transfer of the viral genome and accessory proteins from infected to non-infected cells. I intend to address dynamic interactions of viral and cellular protein complexes leading to membrane curv ature perturbations in their native environment using cryo electron tomography and complementary approaches. We will concentrate on three major systems - (i) Viral and eukaryotic fusion proteins, (ii) cell-cell-transmission of retroviruses and (iii) cellular membrane coats - and study for each of them a particular aspect of membrane curvature modulation at various levels of detail and complexity. We will take advantage of the properties and specificities of the individual systems to understand t he underlying mechanistic properties of membrane remodelling. Furthermore, computational and imaging tools will be developed for the efficient analysis of the structural interactions of the macromolecules. The combination of systems and approaches ultimately aims to integrate high resolution structural information with live cell imaging to achieve a spatio-temporal description of complex biological processes thereby revealing native structure-function relationships.
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.
Grant Details
Amount Awarded | 113032 |
Applicant Surname | Grünewald |
Approval Committee | Science Enhancement Committee |
Award Date | 2014-02-03T00:00:00+00:00 |
Financial Year | 2013/14 |
Grant Programme: Title | Enhancement |
Internal ID | 090895/Z/09/A |
Lead Applicant | Prof Kay Grünewald |
Partnership Value | 113032 |
Planned Dates: End Date | 2015-08-31T00:00:00+00:00 |
Planned Dates: Start Date | 2014-03-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | South East |
Sponsor(s) | Prof David Stuart |