Assembly of infectious rotavirus from cDNA clones: its application to determine the roles of non-structural proteins in virus replication. (360G-Wellcome-093007_Z_10_Z)

Rotavirus is a member of the Reoviridae family of viruses; it has a multilayered capsid and a segmented double-stranded RNA (dsRNA) genome. Like other members of this family it has proven difficult to develop reverse genetics (RG) approaches for this virus. The major aim of this project is to develop a RG system for Rotavirus with a systematic approach. This will be built on a RG method established in our laboratory with Bluetongue virus (BTV), a related dsRNA virus, and further facilitated by t he knowledge and reagents that are already generated for this project application (see preliminary data). Although challenging, it is quite feasible for us to transfer the novel approach that has been highly successful for BTV, and developed a similar RG systems based entirely on T7 RNA transcripts for RV synthesized in vitro. We will generate targeted mutant viruses focussing on three functionally related non-structural proteins that are involved either in rotavirus replication platform or intr acellular trafficking, egress and pathogenesis. Complementing stable cell lines expressing these proteins will be generated to rescue mutant viruses. Together, it will be possible to address some of the outstanding fundamental questions regarding virus replication and pathogenesis of this human (and animal) pathogen.

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Grant Details

Amount Awarded 161539
Applicant Surname Roy
Approval Committee Immunology and Infectious Disease Funding Committee
Award Date 2010-10-05T00:00:00+00:00
Financial Year 2010/11
Grant Programme: Title Project Grant
Internal ID 093007/Z/10/Z
Lead Applicant Prof Polly Roy
Partnership Value 161539
Planned Dates: End Date 2013-12-31T00:00:00+00:00
Planned Dates: Start Date 2011-07-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London