The role of metabolic enzymes in the nucleus (360G-Wellcome-093735_Z_10_A)

£45,000

Cells exposed to oxidants re-route their main carbon flow from glycolysis to the pentose phosphate pathway (PPP), by two different mechanisms. One bases on a biochemical block and operates purely on a metabolic level, the other on transcriptional regulation. We found that the two processes compartmentalize over time: After contact with the stressor, the cell induces the metabolic transition in seconds-timescale but it takes minutes until transcripts raise. Remarkably, metabolic changes seem cau sally implicated in regulating this process: induction of the transition activates transcripts of the antioxidant-system. It is the aim of this proposal to identify and analyze regulatory mechanisms which surround the involved metabolic pathways on a genome-scale level. For this, we will develop multiple reaction monitoring (MRM) assays that allow fast quantification of metabolic intermediates, and screen an entire yeast knock-out library composed of 5,200 systematically generated yeast mutants to identify all gene deletions which cause specific changes in the concentration of their intermediates. In a second step, by using a targeted proteomics strategy, these mutants will be screened to identify alterations in the abundance of implicated enzymes. The identified genetic/metabolome interactions are studied for their general importance on metabolism, and analyzed in respect of yeast aging phenotypes.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 45000
Applicant Surname Ralser
Approval Committee Science Enhancement Committee
Award Date 2014-12-08T00:00:00+00:00
Financial Year 2014/15
Grant Programme: Title Enhancement
Internal ID 093735/Z/10/A
Lead Applicant Prof Markus Ralser
Partnership Value 45000
Planned Dates: End Date 2016-04-30T00:00:00+00:00
Planned Dates: Start Date 2015-01-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England
Sponsor(s) Prof Stephen Oliver