A facility for in-house analysis and X-ray data collection of crystals with large subunit cells. (360G-Wellcome-099145_Z_12_Z)

£394,250

Studies in groups in the Division are aimed at determining structures of large protein complexes using a combination of EM and X-ray crystallography. In the Wigley lab work is focused on several large multi-subunit protein complexes involved in the DNA double-strand break repair process. These include systems that remodel chromatin (Ino80), exchange histones (Ino80 & Swr1) and label them (Tip60 & NuA4). The broken DNA ends are processed by RecBCD/AddAB complexes in bacteria or by interaction s between the Blm/Dna2/RPA, Top3/Rmi1/Rmi2 and Mre11/Rad50/Nbs1 complexes in humans. The Barford lab is studying protein complexes involved in regulation of the cell cycle. A particular interest is the anaphase-promoting complex (APC/C) that functions as an E3 ubiquitin ligase to regulate defined cell cycle transition by targeting specific cell cycle regulatory proteins such as cyclins for degradation through the ubiquitin-proteasome system. The APC/C-co-activator complex comprises 14 differ ent proteins with a molecular mass of 1.2 MDa. The Vannini lab (starting March 2012) will be focussing on RNA polymerase III using a combination of cryo-EM and X-ray crystallography. The Guettler lab (starting October 2012) will be focussing on mechanisms of poly-ADP ribosylation.

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Grant Details

Amount Awarded 394250
Applicant Surname Wigley
Approval Committee Multi-User Equipment Committee (inactive)
Award Date 2012-05-22T00:00:00+00:00
Financial Year 2011/12
Grant Programme: Title Multi-User Equipment Grant
Internal ID 099145/Z/12/Z
Lead Applicant Prof Dale Wigley
Other Applicant(s) Prof David Barford
Partnership Value 394250
Planned Dates: End Date 2015-04-30T00:00:00+00:00
Planned Dates: Start Date 2013-06-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London