INTRAHOST DYNAMICS IN TREATED AND UNTREATED MALARIA PATIENTS. (360G-Wellcome-099992_Z_12_Z)

Plasmodium falciparum malaria infections in sub-Saharan Africa often consist of mixtures of different parasite genotypes. While current methods for genotyping infections can distinguish whether or not multiple parasite genotypes are present, they fail to resolve haplotypes present. This limits our ability to understand multiple-haplotype infections (MIs) which are thought to play key roles in disease severity, drug resistance evolution and malaria transmission. I aim to develop a Fluorescence Ac tivated Cell Sorting (FACS) method to examine the genetic composition of MIs from an area of intense malaria transmission in Malawi. I will isolate component clones of MIs using FACS or limiting dilution cloning and genotype these at 384 genome-wide polymorphic SNPs to determine the number and relatedness of parasite haplotypes within MIs. These data will be used to assess the relationship between within-host parasite genetic diversity and malaria severity, and to understand how malaria superinf ection is regulated in high transmission regions. I also propose to examine the impact of MIs on our ability to detect emerging drug-resistant malaria in the field, and to evaluate the suitability of an in vitro assay for measuring parasite susceptibility to artemisinin, a key antimalarial drug in sub-Saharan Africa.

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Grant Details

Amount Awarded 450000
Applicant Surname Nkhoma
Approval Committee International Interview Committee
Award Date 2013-02-26T00:00:00+00:00
Financial Year 2012/13
Grant Programme: Title International Intermediate Fellowship
Internal ID 099992/Z/12/Z
Lead Applicant Dr Standwell Nkhoma
Partnership Value 450000
Planned Dates: End Date 2017-05-31T00:00:00+00:00
Planned Dates: Start Date 2013-06-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region North West
Sponsor(s) Prof Robert Heyderman, Prof Stephen Ward