Wolbachia transinfection of Culex tritaeniorhynchus mosquitoes to impact transmission of Japanese encephalitis virus . (360G-Wellcome-101285_Z_13_Z)

Transinfection of Drosophila Wolbachia strains in Culex tritaeniorhynchus, which grow to high densities in insect hosts, would likely provide strong protection against Japanese encephalitis virus (JEV), which is part of the same genus of viruses as dengue (Flavivirus). The proposed research would involve introducing Drosophila Wolbachia strains using embryonic microinjection into Culex tritaeniorhynchus, which does not harbour natural Wolbachia infections. Selection for stable transinfected li nes would be followed by an assessment of Wolbachia density and tissue tropism in female mosquitoes. Stable Wolbachia-infected lines would be outbred to maintain a diverse mosquito genetic background. Vector competence of Wolbachia-transinfected lines would be assessed by oral feeding mosquitoes with JEV-spiked bloodmeals at different virus titers. The JEV infection levels in mosquito body parts would be measured and an assessment of infectious JEV virus in the saliva would be undertaken. Se veral strains of JEV would be tested to ensure any effects on transmission would be effective against a diverse panel of JEV strains. The fitness of transinfected mosquito lines would also be measured in addition to large cage invasion experiments to determine the suitability of particular strains for the applied biocontrol use in field populations.

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Grant Details

Amount Awarded 744022
Applicant Surname Walker
Approval Committee Sir Henry Dale Fellowship Interview Committee
Award Date 2013-05-29T00:00:00+00:00
Financial Year 2012/13
Grant Programme: Title Sir Henry Dale Fellowship
Internal ID 101285/Z/13/Z
Lead Applicant Dr Thomas Walker
Partnership Name Royal Society/Wellcome Trust Sir Henry Dale Fellowship
Partnership Value 744022
Planned Dates: End Date 2022-08-01T00:00:00+00:00
Planned Dates: Start Date 2014-02-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London
Sponsor(s) Prof Rodney Phillips