Identification of novel antibiotics from bacteria using combined proteomics and computational approaches (360G-Wellcome-102414_Z_13_Z)
The aim of the project is to discover novel antibiotics from bacterial species, in particular from lactic acid bacteria (LAB) family. Lanthionine-containing antibiotic (lantibiotic) are a class of bacteriocins produced by LAB family that undergo post-translational modifications (PTMs). They have been widely used in the food safety and preservation industry (ex. Nisin) and possess potential biomedical applications. This project will involve Mass Spectrometry (MS) analyses in identification oflantibiotics from LAB species. Sample preparation for MS is a key step for a successful analysis, and two different analyses will be performed: (i) Unlabelled analysis and (ii) Labeling analysis using 2-mercaptoehtanol, sodiumborohydride and ethanethiol. MS analysis software, MaxQUANT, will be modified to incorporate lantibiotic associated modifications such as lanthionine, beta-methyl lanthionine, lysinoalanine, etc. Using genome mining on known lantibiotic associated genes, novel species of LAB will be predicted and analysed using MS. Lantibiotics purified from these species will be assessed
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Grant Details
| Amount Awarded | 155242 |
| Applicant Surname | Maheshwari |
| Approval Committee | PhD Studentships |
| Award Date | 2013-06-24T00:00:00+00:00 |
| Financial Year | 2012/13 |
| Grant Programme: Title | PhD Studentship (Basic) |
| Internal ID | 102414/Z/13/Z |
| Lead Applicant | Mr Nikunj Maheshwari |
| Partnership Value | 155242 |
| Planned Dates: End Date | 2017-08-31T00:00:00+00:00 |
| Planned Dates: Start Date | 2013-09-01T00:00:00+00:00 |
| Recipient Org: Country | Ireland |
| Region | Ireland |
| Sponsor(s) | Prof Geraldine Butler |