Wellcome Trust PhD Programme for Clinicians at the University of Cambridge. (360G-Wellcome-103392_Z_13_Z)

£328,106

Human development progresses from a single fertilised egg to a multicellular organism composed of cells highly-organised into functional units called organs. All the various cells are generated from the same precursor and the processes by which the different cell types emerge is poorly understood. The chromatin structure, transcriptome and proteome may uniquely define each cell subtype. The ranscriptome provides a useful surrogate for the proteome the most downstream affector of function yet may be readily measured due to the favourable chemical properties of nucleic acids allowing amplification with high fidelity. Previous work has allowed us to use single cell qPCR to infer a branched developmental trajectory for Flk1+ cells of the developing mouse mesoderm over a period of developmental time. This work has led to the synthesis of a boolean network, predictions from which were subsequently validate experimentally. The inferred developmental trajectory and network were constructed from a biased choice of genes, a more transcriptome wide unbiased approach has the potential to reveal novel branches not currently identified. The current project aims to reveal the branching hierarchy of the Flk1+ mesoderm at greater resolution and to include more lineage decisions since the choice of genes for the qPCR was specific to the haematopoietic lineages. These branches and network interactions will give new insights to critical fate decisions at bifurcation points and provide a systematic approach to constructing protocols for directed differentiation of pluripotent cells.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 328106
Applicant Surname Jawaid
Approval Committee PhD Studentships
Award Date 2013-09-16T00:00:00+00:00
Financial Year 2012/13
Grant Programme: Title PhD Training Fellowship for Clinicians
Internal ID 103392/Z/13/Z
Lead Applicant Dr Wajid Jawaid
Partnership Value 328106
Planned Dates: End Date 2017-09-30T00:00:00+00:00
Planned Dates: Start Date 2013-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England
Sponsor(s) Prof Krishna Chatterjee