Investigation of an essential enzyme involved in sugar processing in Mycobacterium tuberculosis (360G-Wellcome-104193_Z_16_A)

£94,809

There is currently a lack of understanding of the role of the sugar transport systems in Mycobacterium tuberculosis. The aim of the project is to elucidate the role of these sugar transporters in Mtb by undertaking an interdisciplinary state-of-the-art biochemical, chemical and genetic approach. In this project proteins involved in the uptake of sugars will be recombinantly expressed with the goal of biochemically characterizing the specific substrates that bind and determining the crystal struc ture of these proteins in complex with their physiological ligands, with a view to developing inhibitors of these systems. In parallel a series of mutants of genes involved in sugar uptake will be generated in order to assess the functional role of the transporters and the effects of these mutations determined in vitro by tracking the accumulation of sugars and their incorporation into the cell wall of mycobacteria and in vivo in mice to understand their role in virulence and infection. The mai n goals are: 1) Biochemical characterization and structural determination of the enzymes involved in sugar uptake. 2) Assign physiological functions to genes involve in sugar transport by generating null and conditional mutants in mycobacteria.

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Grant Details

Amount Awarded 94809
Applicant Surname Fullam
Approval Committee Science Enhancement Committee
Award Date 2016-02-11T00:00:00+00:00
Financial Year 2015/16
Grant Programme: Title Enhancement
Internal ID 104193/Z/16/A
Lead Applicant Dr Elizabeth Fullam
Partnership Value 94809
Planned Dates: End Date 2018-08-31T00:00:00+00:00
Planned Dates: Start Date 2016-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region West Midlands