Focussed ion beam and scanning electron microscope for 3D reconstruction of large volumes in neuro- and cell biology and nano engineering of electron cryotomography samples. (360G-Wellcome-104905_Z_14_Z)

£354,461

We are proposing to acquire a FIB/SEM dualbeam instrument at the MRC Laboratory of Molecular Biology in Cambridge (LMB). LMB will provide dedicated, newly fitted-out facility space, staff support, a service contract and half of the instrument cost. Access of the wider community is ensured through the inclusion of Cambridge University's imaging centre and our dedication to make up to 30 % of time available to outside users. The focussed ion beam (FIB) is exceptionally useful for the removal of ma terial from samples for subsequent electron microscopy, at sub 5 nm resolutions not achievable by any other means. The machine will have two principal uses: firstly, the FIB will be used to serially remove very thin layers from large blocks of embedded tissues, with the electron beam producing images of each layer. This will allow automatic three-dimensional reconstruction of large volumes of whole tissues, making it possible to trace neurons and their connections or sub-cellular membrane domain s (FIB/SEM 'slice-and-view'). Secondly, milling of samples for electron cryotomography will enable us to visualise the interior of cells at isotropic resolutions approaching individual molecules and this application of the instrument will make heavy use of LMB's recent investment in high-resolution electron cryomicroscopy.

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Grant Details

Amount Awarded 354461
Applicant Surname Löwe
Approval Committee Multi-User Equipment Committee (inactive)
Award Date 2014-06-25T00:00:00+00:00
Financial Year 2013/14
Grant Programme: Title Multi-User Equipment Grant
Internal ID 104905/Z/14/Z
Lead Applicant Dr Jan Löwe
Other Applicant(s) Dr Jeremy Skepper, Prof Tiago Branco
Partnership Value 354461
Planned Dates: End Date 2017-11-30T00:00:00+00:00
Planned Dates: Start Date 2014-12-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region South West