FILM microscopy facility: New generation confocal and high content screening microscopes, and upgrades for intravital microscopy. (360G-Wellcome-104931_Z_14_Z)
For the systematic investigation of contextual physiological information, we request a High-content screening (HCS) microscope with confocal fluorescence capacity. FILM already supports experimental upscaling and data analysis of HCS screens performed elsewhere, but increased capacity and direct access to a confocal HCS system are urgently needed. FILM has an outstanding track record in intravital microscopy, but a major limitation is currently detector sensitivity and multiphoton laser power . We therefore request an upgrade of the intravital microscope with hybrid detectors and DeepSee laser to increase imaging depth. Several users perform research on the effects of mechanical cell stress. The Flexcell system would allow microscopic measurements under controlled conditions including the imaging of fluorescence biosensors which readout the intracellular signalling consequences of mechanical strain. FILM embeds leading-edge technologies into a full support system offering acces s to a large range of high-end microscopy technologies. Some of our microscopes are outdated with regard to speed and sensitivity, we therefore request funds to replace one ageing confocal by a new-generation system. All these technologies critically depend on expert support, and the multisite nature of Imperial College makes constant support difficult. We request an additional support staff position to cope with increased and distributed demand.
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Grant Details
Amount Awarded | 590993 |
Applicant Surname | Magee |
Approval Committee | Multi-User Equipment Committee (inactive) |
Award Date | 2014-06-25T00:00:00+00:00 |
Financial Year | 2013/14 |
Grant Programme: Title | Multi-User Equipment Grant |
Internal ID | 104931/Z/14/Z |
Lead Applicant | Prof Anthony Magee |
Other Applicant(s) | Dr Nadia Guerra, Dr Vania Braga, Prof Cristina Lo Celso, Prof Mark Griffiths, Prof Sian Harding |
Partnership Value | 590993 |
Planned Dates: End Date | 2021-01-31T00:00:00+00:00 |
Planned Dates: Start Date | 2014-10-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Greater London |