FILM microscopy facility: New generation confocal and high content screening microscopes, and upgrades for intravital microscopy. (360G-Wellcome-104931_Z_14_Z)

£590,993

For the systematic investigation of contextual physiological information, we request a High-content screening (HCS) microscope with confocal fluorescence capacity. FILM already supports experimental upscaling and data analysis of HCS screens performed elsewhere, but increased capacity and direct access to a confocal HCS system are urgently needed. FILM has an outstanding track record in intravital microscopy, but a major limitation is currently detector sensitivity and multiphoton laser power . We therefore request an upgrade of the intravital microscope with hybrid detectors and DeepSee laser to increase imaging depth. Several users perform research on the effects of mechanical cell stress. The Flexcell system would allow microscopic measurements under controlled conditions including the imaging of fluorescence biosensors which readout the intracellular signalling consequences of mechanical strain. FILM embeds leading-edge technologies into a full support system offering acces s to a large range of high-end microscopy technologies. Some of our microscopes are outdated with regard to speed and sensitivity, we therefore request funds to replace one ageing confocal by a new-generation system. All these technologies critically depend on expert support, and the multisite nature of Imperial College makes constant support difficult. We request an additional support staff position to cope with increased and distributed demand.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 590993
Applicant Surname Magee
Approval Committee Multi-User Equipment Committee (inactive)
Award Date 2014-06-25T00:00:00+00:00
Financial Year 2013/14
Grant Programme: Title Multi-User Equipment Grant
Internal ID 104931/Z/14/Z
Lead Applicant Prof Anthony Magee
Other Applicant(s) Dr Nadia Guerra, Dr Vania Braga, Prof Cristina Lo Celso, Prof Mark Griffiths, Prof Sian Harding
Partnership Value 590993
Planned Dates: End Date 2021-01-31T00:00:00+00:00
Planned Dates: Start Date 2014-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London