The molecular mechanism of virulence effector protein translocation by Salmonella SipB. (360G-Wellcome-105390_Z_14_Z)

£160,792

A critical step in pathogenesis of enteric bacteria such as Salmonella is hijacking the host cell. This event depends on the type three secretion system (T3SS) which enables the delivery of subversive virulence effectors into the host 3,11. A hydrophobic protein called SipB is part of the T3SS and forms the translocon element 3. While the mechanism of effector translocation across the host cell plasma membrane remains elusive, previous work in our lab revealed that SipB is essential for translocation and has liposome fusion activity 7. When fusion is inhibited with a truncated derivative of SipB (SipB428-593), Salmonella entry into host cells is prevented6. The aim of this project is to build upon this foundation to research the mechanism of effector delivery into host cells. We will use established in vitro liposome fusion assays – together with biochemical reconstitution and high-resolution structural studies – to reveal the mechanism of translocation. Following the successful work with SipB 428-593, we aspire to screen natural extracts and synthetic compound libraries in search of a T3SS inhibitor, which could have therapeutic value. Finally, we will also extend our work onto homologous T3S systems from other pathogens, such as Shigella, Yersinia and E. coli.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 160792
Applicant Surname Wolanska
Approval Committee PhD Studentships
Award Date 2014-07-14T00:00:00+00:00
Financial Year 2013/14
Grant Programme: Title PhD Studentship (Basic)
Internal ID 105390/Z/14/Z
Lead Applicant Ms Dominika Wolanska
Partnership Value 160792
Planned Dates: End Date 2018-09-30T00:00:00+00:00
Planned Dates: Start Date 2014-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England
Sponsor(s) Prof Paul Lehner