The impact of stress on corticosteroid receptor binding to glucocorticoid target genes in the rat brain in relation to gene transcriptional responses and glucocorticoid hormone secretion (360G-Wellcome-109068_Z_15_A)

£0

My project concerns investigations on the action of stress-induced glucocorticoid hormones (GCs) in the rat brain. GCs play a critical role in an organism’s ability to adapt to external challenges and are crucial for maintaining health and wellbeing. Recently, the Reul lab demonstrated the enhanced binding of mineralocorticoid (MRs) and glucocorticoid (GRs) receptors to GC-responsive elements (GREs) within the GC-target genes Fkbp5 (FK506-binding immunophilin 51), Per1 (Period1), and Sgk1 (serum- and GC-inducible kinase 1)) in the rat hippocampus. My project will compare MR and GR to GRE binding within these genes in the hippocampus, neocortex, prefrontal cortex, amygdala, and hypothalamus, and pituitary under baseline and stress conditions and correlate these findings with the respective RNA levels generated under such conditions. In adrenalectomized (ADX) rats the separate and combined role and contribution of MRs and GRs to GRE-binding and transcriptional effects will be studied using pharmacological approaches. Bioinformatics analysis will be conducted on data sets generated by MR and GR ChIP-seq and RNA-seq on hippocampus of baseline and stressed rats. These analyses will involve comparison of MR/GR GRE-binding across the genome, comparison of these GRE-binding profiles with in silico generated data, and cross-correlational analysis if the ChIP-seq and RNA-seq data sets.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 0
Applicant Surname Kennedy
Approval Committee Internal Decision Panel
Award Date 2017-01-31T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title PhD Studentship (Basic)
Internal ID 109068/Z/15/A
Lead Applicant Miss Clare Kennedy
Partnership Value 0
Planned Dates: End Date 2019-09-30T00:00:00+00:00
Planned Dates: Start Date 2016-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region South West