Molecular basis for heterochromatin formation in the African trypanosomes (360G-Wellcome-109092_Z_15_A)

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Histones are some of the most well-conserved proteins between eukaryotic species and specific post-translational histone modifications often trigger the same cellular responses in different organisms. However, the histones of Trypanosoma brucei, the causative agent of sleeping sickness, are more divergent than the histones in other organisms. In most eukaryotes, heterochromatin is formed by adding methyl groups to Lys9 of histone H3. Trypanosomes lack the H3K9 residue and heterochromatin formation in these parasites must be specified by a distinct mechanism. This project aims at uncovering the histone modifications that mediate trypanosome heterochromatin formation. Next-Generation mass spectrometry analysis will be performed to survey histone modifications in the different developmental forms of T.brucei. Additionally, the composition of the trypanosome heterochromatin will be characterised using several alternative strategies including: fluorescent tagging of putative readers, writers and erasers of heterochromatin-associated histone modifications; isolating trypanosome nuclei and enriching for lamina-associated chromatin; and CRISPR/Cas9 or TAL targeting of GFP to repetitive elements in T.brucei.

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Grant Details

Amount Awarded 0
Applicant Surname Staneva
Approval Committee Internal Decision Panel
Award Date 2017-01-31T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title PhD Studentship (Basic)
Internal ID 109092/Z/15/A
Lead Applicant Ms Desislava Staneva
Partnership Value 0
Planned Dates: End Date 2019-09-30T00:00:00+00:00
Planned Dates: Start Date 2016-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Scotland