Organisation, packaging and transcription of dsRNA viral genomes (360G-Wellcome-109135_Z_15_A)
First, the structure of the first genome layer of ?6 will be revisited with the goal of better separating the different conformations and obtaining a better resolution for each of them. Next, the pathing of the dsRNA and its interactions with the capsid will be studied to understand its organisation and connection to the inner layers at the molecular level. The calculated orientations will then be used to subtract the density of the first layer from the original images in order to examine the inner layers more accurately. The most important reason to continue examining the inner layers is to locate the RNA-dependent RNA polymerases and solve their structures together with the genome. This would facilitate the understanding of how they interact with the dsRNA and, by using inhibitors, different stages in the lifecycle of the virus could be trapped. As a result, a time-resolved mechanism for the packaging of the genome could be proposed. Consequently, the transcription of the dsRNA will be investigated by studying the viruses upon the incubation with a "reaction buffer". Ultimately, the optimized protocol will be applied to study biomedically more relevant viruses such as the human rotavirus.
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