Activity and specificity of Notch regulated enhancers (360G-Wellcome-109144_Z_15_A)
Signalling pathways are required iteratively during development, coordinating many different developmental processes. This diversity relies on them eliciting different qualitative and quantitative transcriptional responses depending on the context. The Notch pathway is one of the small set of core pathways and can promote distinct outcomes through different sets of target genes. How Notch responsive enhancers achieve this level of regulation is not understood. In this project, well characterized Notch responsive enhancers will be used to study how activity and specificity in response to Notch are encoded within the enhancer sequence and how different factors play a role in the control of these properties. 1. Activity: Characterize the transcriptional output of a Notch responsive enhancer and analyze how it is governed. 2. Specificity: Compare two enhancers that exhibit different response to Notch to determine how their response specificity is achieved. 3. Sufficiency: Assess if a defined enhancer is sufficient to confer a specific Notch response when introduced into a heterologous locus.
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.