Spatio-temporally multiplexed 2-photon imaging determining neural coding principles of sensory perception. (360G-Wellcome-109908_Z_15_Z)

How does the brain encode the contents of sensory perception and translate them into meaningful neuronal representations of our environment? Although we know approximately how sensory input is routed through different brain areas, each extracting distinct aspects of the percept, the neuronal language used to represent sensory experiences remains elusive. Our work aims is to unravel the coding strategies employed in the neuronal representation of tactile sensory input. Our key goal is to employ c utting-edge approaches for two-photon microscopy and optogenetic tools to monitor and manipulate neural activity in the neocortex of mice. Combining cell type and circuit specific optical recording and manipulation in studies of awake, behaving mice presents a powerful approach that will permit significant conclusions about general coding principles and allow causal links between neural codes and behavioural output. Support in form of a Seed Award would enable us to implement state-of-the-art te chnology that would permit the recording of neural activity in at least two different depths in the cerebral cortex simultaneously. Data generated with this technology will be key to understanding neural circuit function in different cortical layers and provide preliminary data of high impact for more substantial research programme grant and fellowship applications.