An investigation of the therapeutic potential of a novel marker of tumourigenesis (360G-Wellcome-201745_Z_16_Z)

Aims To establish whether an unusual splicing event of CRMP2 contributes to tumourigenesis. Learn how to carry out scientific research and basic laboratory skills Approaches: I will learn how to culture tumour cells, fractionate sub-cellular components and identify expression of different variants of CRMP2 in each compartment. Further to this, I will learn how to clone different forms of CRMP2 and express these in cells. The project will then move to the generation of novel cell lines where the unusual splice variant of CRMP2 is removed from tumour cells that express it, and increased in cells that do not contain it. This will allow the investigation of the sufficiency and dependency of CRMP2 on tumour cell growth. All of the tools and techniques required are established in the host laboratory.