The molecular mechanism of chromosome condensation mediated by cohesin and condensin (360G-Wellcome-202062_Z_16_Z)
The condensation of chromosomes in the eukaryotic nucleus is essential for life, without it cells cannot faithfully segregate chromosomes. It is well known that the highly conserved condensin and cohesin protein complexes are the key factors for DNA condensation, but the physical mechanism for condensation remains unknown. One possible basis of high-order DNA organisation is that distant DNA segments (within one molecule) interact with each other mediated by cDNA bound condensin and/or cohesin. While extensive methods have been established to study inter-DNA interaction (e.g. sister chromatid cohesion), no techniques have been established to investigate biochemical properties of proposed intra-DNA interactions, required for chromosome condensation. To understand how chromosomes are organised by condensation, it is essential that new technologies are developed, as proposed here. Condensation in Saccharomyces cerevisiae has been studied using repetitive rDNA sequences as a model for conserved DNA condensation. I have established a novel genetic system to permit isolation of condensed chromatin formed in vivo by intra-rDNA interactions. Using Cre recombinase condensed native rDNA discrete chromatin circles can be liberated for biochemical characterisation. These studies will help us formulate a hypothesis on how cohesin/condensin organises rDNA, to build up a solid foundation for further grant application.
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.
Grant Details
Amount Awarded | 99856 |
Applicant Surname | Hu |
Approval Committee | Science Seeds Advisory Panel |
Award Date | 2016-04-08T00:00:00+00:00 |
Financial Year | 2015/16 |
Grant Programme: Title | Seed Award in Science |
Internal ID | 202062/Z/16/Z |
Lead Applicant | Dr Bin Hu |
Partnership Value | 99856 |
Planned Dates: End Date | 2018-10-02T00:00:00+00:00 |
Planned Dates: Start Date | 2016-10-03T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Yorkshire and the Humber |