The molecular mechanism of chromosome condensation mediated by cohesin and condensin (360G-Wellcome-202062_Z_16_Z)

£99,856

The condensation of chromosomes in the eukaryotic nucleus is essential for life, without it cells cannot faithfully segregate chromosomes. It is well known that the highly conserved condensin and cohesin protein complexes are the key factors for DNA condensation, but the physical mechanism for condensation remains unknown. One possible basis of high-order DNA organisation is that distant DNA segments (within one molecule) interact with each other mediated by cDNA bound condensin and/or cohesin. While extensive methods have been established to study inter-DNA interaction (e.g. sister chromatid cohesion), no techniques have been established to investigate biochemical properties of proposed intra-DNA interactions, required for chromosome condensation. To understand how chromosomes are organised by condensation, it is essential that new technologies are developed, as proposed here. Condensation in Saccharomyces cerevisiae has been studied using repetitive rDNA sequences as a model for conserved DNA condensation. I have established a novel genetic system to permit isolation of condensed chromatin formed in vivo by intra-rDNA interactions. Using Cre recombinase condensed native rDNA discrete chromatin circles can be liberated for biochemical characterisation. These studies will help us formulate a hypothesis on how cohesin/condensin organises rDNA, to build up a solid foundation for further grant application.

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Grant Details

Amount Awarded 99856
Applicant Surname Hu
Approval Committee Science Seeds Advisory Panel
Award Date 2016-04-08T00:00:00+00:00
Financial Year 2015/16
Grant Programme: Title Seed Award in Science
Internal ID 202062/Z/16/Z
Lead Applicant Dr Bin Hu
Partnership Value 99856
Planned Dates: End Date 2018-10-02T00:00:00+00:00
Planned Dates: Start Date 2016-10-03T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Yorkshire and the Humber