Laser capture microdissection to investigate bacterial handling defects in patients with monogenic inflammatory bowel disease (360G-Wellcome-202232_Z_16_Z)
Inflammatory bowel disease (IBD) is linked to defective bacterial handling in Crohn’s disease and monogenic diseases (Hugot et al. Nature 2001, Ogura et al. Nature 2002, Cooney et al. Nature Medicine 2010, Uhlig Gut 2013 and Uhlig et al. Gastroenterology 2014). Hypothesis: If defects in clearance of ingested bacteria cause granuloma formation we expect to find surviving bacteria (and its mRNA) in the granuloma lesions. Experimental Design Formalin-fixed archived tissue sections from patients with monogenic forms of IBD (XIAP , G6PC3 or HPS1) will be cut onto a capture membrane and stained with H & E. Granuloma will be isolated using laser capture micro dissection. 16S rRNA will be amplified using high fidelity PCR and sequenced. Comparison of the obtained sequences with public databases will establish which bacterial taxa. This project will help to identify bacteria that survive within macrophages and improve the understanding of the host-environment relationship.
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Grant Details
Amount Awarded | 2000 |
Applicant Surname | Rutenberg-Houchen |
Approval Committee | Internal Decision Panel for C&S |
Award Date | 2016-04-01T00:00:00+00:00 |
Financial Year | 2015/16 |
Grant Programme: Title | Vacation Scholarships |
Internal ID | 202232/Z/16/Z |
Lead Applicant | Mr Kristian Rutenberg-Houchen |
Partnership Value | 2000 |
Planned Dates: End Date | 2016-08-19T00:00:00+00:00 |
Planned Dates: Start Date | 2016-06-20T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | South East |