An electron cryo-microscopy resource for macromolecular structure determination in the University of Cambridge (360G-Wellcome-202905_Z_16_Z)

£1,000,000

We seek to establish an advanced electron cryo-microscopy (cryo-EM) facility dedicated to structural studies of biological macromolecular assemblies. The facility would provide a revolutionary new tool to the large structural biology community in the University that would enable acquisition of critical data in support of a wide and diverse range of projects tackling fundamental problems in molecular biology relevant to human health. Currently, the named applicants primarily use X-ray crystallography to study large assemblies, but many of these samples cannot be readily crystallised. The recent development of a new generation of direct electron detectors, together with sophisticated data-processing software, has dramatically improved cryo-EM analysis, which now achieves routinely sub-nanometer resolution. However, we lack access to such a facility in the University. A wide range of projects will benefit from the proposed facility, including studies of multi-drug efflux membrane transporters; assemblies of RNA metabolism; complexes regulating chromatin structure, DNA replication and repair; mitochondrial complex I and its roles in human disease; ribosome assembly defects in bone marrow failure; presenilin complexes and other assemblies related to neurodegeneration; human neurotransmitter transporters and G-protein coupled receptors; transport vesicle and organelle biogenesis; molecular mechanism of ATP synthase; and methods development in EM of biological macromolecules.

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Grant Details

Amount Awarded 1000000
Applicant Surname Luisi
Approval Committee Multi-User Equipment and Technology Development Committee
Award Date 2016-06-16T00:00:00+00:00
Financial Year 2015/16
Grant Programme: Title Multi-User Equipment Grant
Internal ID 202905/Z/16/Z
Lead Applicant Prof Ben Luisi
Other Applicant(s) Dr Judy Hirst, Dr Simone Weyand, Prof Alan Warren, Prof David Owen, Prof Ernest Laue, Prof Luca Pellegrini, Prof Peter St George-Hyslop, Prof Randy Read, Prof Sir John Walker
Partnership Value 1000000
Planned Dates: End Date 2021-12-19T00:00:00+00:00
Planned Dates: Start Date 2016-12-19T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England