DNA repair status and drug sensitivity of a novel patient derived cell line (360G-Wellcome-206723_Z_17_Z)

In this project I will measure the expression of various proteins involved in the DNA damage response (DDR) in extracts of a novel cell line that spontaneously immortalised from a primary culture of ascites cells from a patient with clear cell ovarian cancer. I will also determine the sensitivity of this cell line to DNA damaging anticancer drugs and ionising radiation, potentially also in combination with inhibitors of the DDR, e.g., ATR, PARP, using both growth inhibition and cytotoxicity assays. If time allows, I will also measure cell cycle perturbations after DNA damage. At the end of the project I will compare the cytotoxicity data with the protein expression data (and potentially the cell cycle analysis) to determine if expression levels of DDR proteins can be related to sensitivity.